Sentence examples for necessitates the expression from inspiring English sources

This step necessitates the expression of genes involved in the Bone Morphogenetic Protein (Bmp) signaling pathway (in mammals, Bmp2 and Bmp4) which activates cell differentiation, but also the Hedgehog and Fibroblast Growth Factor (Fgf) signaling pathways which induce proliferation and counteract the Bmp pathway [ 7– 10].

Of note was the fact that increased LN dissemination necessitated the expression of RasG12V in the cells as well as supplementation of CM derived from cells expressing hyper-activated Ras and stimulated by TNFα (=CMRasG12V+TNFα).

This typically necessitates mutagenesis of the expression construct to increase protein yield [ 4] plus the incorporation of additional mutations to stabilise the resultant protein so it is more likely to crystallise [ 3].

Such liberty necessitates the discussion and expression of ideas that may be unpalatable to others.

The employment of different bacterial species as expression systems therefore necessitates the acquisition or development of new expression vectors [1], [2].

In addition, comparative analyses of primate mitochondrial genomes show that predicted antisense antitermination tRNA properties coevolve positively with putative mitochondrial overlapping genes (including gau) whose expression necessitates the suppressor/antitermination antisense tRNAs [ 5].

One difference to the CMAP database is necessitated by the multiple origins of the expression profile data represented by multiple probe ID definitions.

This hypothesis is further supported by the observation that CK+ CTCs in patients with early stage breast cancer are more heterogeneous with regard to the expression of EMT markers, necessitating additional studies to further elucidate their distinct biological role.

Our work have emphasized the importance of protein-complex forming propensity of the proteins in addition to their connectivity in the protein-protein interaction network as the key underlying force guiding their evolutionary rates and necessitating the increase in expression level for the DF and SP proteins.

However, retrieval of most relevant biological networks/pathways associated with the upper or lower end of the data distribution is not a trivial task, mainly because members of a biological pathway do not usually have similar data values (e.g. gene expression change), which necessitates the use of various computational algorithms for finding such networks of genes [ 1- 3, 6- 9].

Our decision to use RACE to verify the expression and structure of these un-annotated genes was necessitated by their low level of expression and uncertain gene structures.