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This, in addition to identification of both single-point substitutions and small deletions, as well as a 2.2 kb deletion, necessitates screening of the entire genes both for single-point mutations and small insertions/deletions as well as large genomic rearrangements.
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Because reliable methods to characterize recalcitrance of plant cell walls to saccharification do not yet exist, identification of superior biomass species for ethanol production necessitates screening deconstruction of lignocellulosic biomass by pretreatment and subsequent enzymatic hydrolysis.
Identifying viruses currently necessitates screening with a wide panel of immunological or molecular probes and may be best done by a specialist testing service.
In some cases, difficulty of detecting A T ↔ T A mutations in 6-fold pools necessitated screening all of the 288 fish in a pool individually by HRM.
The substantial number of false positive colonies resulting from this second selection step, necessitates the screening of up to hundreds of colonies before obtaining the correctly recombineered end-product (Supplement Table S1).
Proponents argue that IPV's burden of suffering necessitates universal screening of all women presenting to healthcare settings [ 17, 18] with the hope that this will lead women on a path of help-seeking, with eventual reductions in violence and its health consequences.
The criteria for enrolment are strict and well-defined, necessitating the screening of large numbers of individuals for each subject enrolled.
When we began working with tephritid genomes, we viewed their large size as an annoyance that necessitated the screening of an unusually large (compared to Drosophila) number of clones to identify genes of interest.
Population-based trials of H. pylori eradication are feasible but necessitate screening large numbers of people to identify those who are infected and who may benefit from eradication.
The few reports to date of mutagenesis of S. suis used allele exchange without a counterselection marker and necessitated the laborious screening of large numbers of colonies (24– 24).
However, CHO cells also readily amplify their own GS gene, necessitating the isolation and screening of single clones, adding 1 2 months to the generation of a high-expressing cell line.
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