As of this writing Gregory Logan, now 60 years old, is incarcerated in a federal prison in Pennsylvania, where he is facing an uncomfortable stay—his law enforcement past will necessitate isolation from his fellow convicts.
Analysing cells of one type, such as monocytes[1], [2], [3], necessitates an isolation step that may be technically difficult[4] and which may induce non-specific changes in gene expression.
When Colombia resumed extradition in 1997, the system was designed for powerful drug dons, guerrilla commanders and paramilitary leaders wanted in the U.S. The detainees tended to be extremely dangerous and well connected, necessitating the isolation.
However, CHO cells also readily amplify their own GS gene, necessitating the isolation and screening of single clones, adding 1 2 months to the generation of a high-expressing cell line.
Hence, the use of MSC as cellular therapeutics necessitates stan-dardized isolation and reliable quality control of cell preparations.
Hence, we predict that the occurrence of limits to host range in spider mites necessitates periods of isolation on different host plants.
Therefore, the blocking failure of one arm of the converter and the whole converter blocking failure are both considered as emergency condition, necessitating the fault isolation with the special control and protection strategy proposed in Section 4.1.
The differential enhancement of the tumor in this case is not amenable for semi-automated isolation, necessitating the use of additional intravenous contrast phases, or fusion with other imaging such as MRI or PET where the tumor extent may be more accurately demonstrated, per the discretion of the radiologist and the clinical team.
The acute nature of the recent outbreak necessitated imposing quarantine, isolation, and other restrictive measures, in addition to monitoring movement of travellers.
The inability to distinguish the MVA-BAC clones by restriction map, viral growth yield or murine immunogenicity suggests that they are likely to be identical, but the possibility of some bias in the isolation procedure necessitates verification of clone fidelity by complete sequencing, especially prior to embarking on a programme of genetic manipulation.
