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However, most used far fewer samples than would be necessitated by using unmatched tissue or stool samples.
The blots were not stripped and re-probed and in the Figures black lines as lane spacers indicate that the lanes have been re-ordered from the same gel to aid clarity; white spaces between lanes reflect separate gels/blots necessitated by using different antibodies.
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Proteins were extracted using the Qproteome FFPE Tissue kit (Qiagen) following the manufacturer's instructions, with modifications necessitated by the use of paraformaldehyde-fixed tissues.
This simplification was necessitated by the use of population balances which require the probability of scission at each chain linkage to be known.
The number of lexicons increased, perhaps necessitated by the growing use of Sanskrit by non-Sanskrit speakers.
The two-lane highway north of Barrie was widened starting in 1977, necessitated by the increasing use of the highway by recreational tourists and vacationers.
The requirement of an antibody with similar affinity for antigen presented in solid or liquid phases is necessitated by its intended use in an inhibition enzyme-linked immuno-sorbent assay (ELISA) depicted in the flow chart shown in Figure 1.
This seismic risk reduction was necessitated by available technology, resource use option and risk management strategies.
Regardless, those of us who care about racing must face head-on the important issues facing the health and well-being of the Thoroughbred namely, the perilous and ill-advised breeding practices that produce speedy yet fragile horses that don't stack up against their sturdier predecessors of the 20th century, and the proliferation of drug use necessitated by the weaknesses of both beast and man.
While several countries have regulations for CSS use, its use is not necessitated by law in Iran.
Comparative analysis and interpretation of seed and charcoal evidence thus illustrates the variety of fuel use strategies necessitated by the dynamic and diverse Bronze Age landscapes of the Eastern Mediterranean.
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