Subgroup analysis produced results using relatively small sample sizes; therefore, these findings necessitate validation on larger patient cohorts.
It is important to note that the small sample size, necessitates validation of these candidate gene expression changes in a larger cohort.
Moreover, high-throughput techniques like microarrays are associated with limitations such as experimental and biological noise that necessitate further validation of the results obtained using other relatively accurate methods.
Unfortunately, the poor aqueous solubility and high membrane binding affinity of hydrophobic drugs necessitates extensive validation of experimental methods to determine both liposome loading and permeability and thus the development of a quantitative understanding of the factors governing the encapsulation and retention/release of such compounds has been slow.
The vast diversity of cellular structures and intra- and extracellular metabolites necessitates the validation of metabolomic sampling protocols for different organisms.
This necessitates experimental validation of enhancer-gene interactions determined using prediction-based methods.
Although these studies are of outstanding quality, they each suffer from relatively small sample sizes, necessitating preliminary validation of proposed gene sets.
This necessitates the validation of biomarkers (e.g., liver enzymes, renal biomarkers, and blood pressure) commonly applied in other populations or exploration to develop, evaluate, and validate new approaches to assess drug effect or side effects (PD) in infants or pregnant women that are valid and appropriate for clinical use.
Molecular diagnostics have, in this respect, the advantage of being both sensitive and easy to integrate in laboratory practice, but the lack of morphological proof of a signal necessitates validation experiments.
Variations in functional conservations and regulatory circuits necessitates a profound validation of biomarker quality, and our study provides a quantitative approach to systematically search for these biomarkers and to evaluate their predictive potential.
