In summary, our results showed that the LD method was broadly applicable to species with iteroparous life histories, although the limited range of life histories considered in our simulation study necessitates caution when the method is applied to estimate N e in species with overlapping generations.
However, applications to practical problems necessitate extensions that enable the method to deal with more complex processes.
Analysing related data and interpreting the results necessitate the use of methods that take this complexity into account.
NASA's anticipated return to the Moon by 2020 and subsequent establishment of a lunar base will necessitate the development of methods to utilize lunar resources for various construction and resource extraction applications.
Importantly, spine surgeons could easily implement this technique as it did not necessitate the learning of methods that were completely foreign to them, nor did it dictate the type of fusion [ 4, 34].
Glycerol is a by-product of many industrial processes and huge amounts of it are generated in the form of waste, thereby necessitating a search for the method of its disposal.
The method only necessitates an estimate of the range of the uncertain variables that encapsulates the true range of the input variables with overwhelming probability.
Issues with traditional EN techniques when applied to field conditions necessitate the need for simplified methods to incorporate the benefits of EN monitoring to in-situ application.
The inherent limitations of antibody-inhibition studies necessitate the use of complementary methods to block integrin function to confirm cell surface interaction.
We demonstrate that these methodological principles necessitate the use of mixed methods, and this case study presents a 'tool-kit' of data generation techniques that produced visual, verbal, textual, technical and scientific data.
Very high resolution (VHR) observations from new sensors will necessitate the development of new methods as the pixel dimensions dip below the size of individual tree crowns, houses, and fields.
