Sentence examples for necessary to reconstitute from inspiring English sources

Very importantly, the use of purified recombinant proteins expressed in E. coli cells suggests that the MHC-I CD and Nef together are sufficient to bind directly to μ1; no additional components or mammalian cellular factors are necessary to reconstitute binding in vitro.

In this new graph, in which all new (sub-) nodes represent subsequences, it is now necessary to reconstitute edges.

This indicates that both variable regions (V H and V L ) are necessary to reconstitute the binding site of an antibody.

Currently, the inability to expand an efficient HBV-specific immunity in a large proportion of adult CHB patients (particularly those with high HBV replication levels) [ 9] might suggest that more radical approaches are necessary to reconstitute a functional efficient HBV-specific T-cell repertoire.

Therefore, to not have two equally intense H{N} HSQC cross-peaks for many backbone amides that are near the heme, it is necessary to reconstitute the NP4 apoprotein with the so-called "symmetrical hemin", 2,4-dimethyldeuterohemin, in which the 2- and 4-vinyl groups of protohemin have been replaced with methyls.

For the CD type, in most cases (230 over 252), the gene encoding the CD was colocalized with that of encoding the corresponding TAD (supplementary tables S2 and S3, Supplementary Material online), consistent with the notion that the presence of both are necessary to reconstitute a fully functional enzyme.

The first functional evidence that TERT comprised the telomerase core was that wild-type hTERT is necessary for telomerase activity in cell extracts [ 10], and that recombinant hTR and hTERT are necessary and sufficient to reconstitute telomerase activity in vitro and in cells [ 17- 19].

Owing to high attrition rates of these volunteers, we found it necessary to train more CHVs in order to reconstitute the community units.

These scaffolds have no known catalytic function and are not required to reconstitute BER in vitro, but are necessary for efficient BER in vivo.

Therefore, to investigate tumor cell behavior ex vivo it is necessary to maintain or reconstitute an environment closely resembling the tumor tissue.

Conde et al. suggest that to study behavior of tumors it is necessary to maintain or reconstitute a similar environment of the tumor in situ [ 29].