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The final portion of the C. difficile CRISPR-Cas9 plasmid is the donor region which is necessary to insert a desired mutation.
In case the block is small the wedges are placed by the workman's hand; it not being necessary to insert them beyond the edge of the block.
George W. Bush could find it necessary to insert itself into private enterprise, the rescue of Fannie Mae and Freddie Mac in September laid that uncertainty to rest.
In every case, they speak for themselves, but in many of his books Steinbeck felt it necessary to insert a little homily on the communal virtues early in the narrative.
In cases in which an allergic reaction is not the underlying cause of the condition, it may be necessary to insert a tiny plastic tube through the membrane to aid in reestablishing normal ventilation of the middle-ear cavity.
If not it may be necessary to insert new management.
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Here we described the necessary maneuvers to insert a DLT using a standard non-channeled blade of King Vision™ VL.
It is not necessary to introduce the entire BAC insert as any particular region can be subcloned into the pBACLink vectors.
Although it is sometimes useful to modify run, it is not necessary or advisable to insert model setup or instrumentation code into this procedure.
Because of the questions she sought answers to, it was absolutely necessary for her to insert herself into this story, so that she could be our moral guide, our pair of eyes and ears, in this altered human landscape.
"The mistrust between the two sides was at such a poisonous level that it was necessary for us to insert ourselves and get them to sign a peace treaty, in effect," said Mr. Tahl, 37, president of Tahl Propp and former associate general counsel at the Trump Organization.
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