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It was necessary to cluster this broad distribution into a number of manageable classes that could be correlated to the genotypes under investigation.
To extract functions that are significantly enriched in one sample over a second dataset, a p-value is calculated based which shows overrepresentation of a specific GO term, thereby it is necessary to cluster related GO-terms.
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In order to conveniently expound and understand the data objects and extract inherent information or knowledge hidden in the data, it is necessary to use cluster analysis technology.
The ten piRNA cutoff value used in the Ae. aegypti study [ 22] was necessary to improve cluster detection when working with such a large dataset.
Although the percentage of hubs necessary to disrupt clusters gradually increased, the percentage necessary was <4% even in clusters of 18 neurons.
It was necessary to change to cluster randomisation with the unit of randomisation being the treating physiotherapist.
This grouping is necessary to maintain clustered and tandemly repeated miRNAs as single units (loci) during the bootstrap procedure.
Genotype calling for each SNP was rechecked manually and rescored if necessary to optimize clustering of progeny categories.
Parameter optimisation of the MCL inflation score (I) is often necessary to maximise clustering performance (the quality of derived GO predictions based on specificity, sensitivity and F-measure).
However, in the case of TB, it is necessary to know which cluster alarms merit further investigation and which clusters are likely to have occurred by chance.
Hemagglutination inhibition assays experimentally indicated that two amino acid changes (156KQ and 158EK) were necessary to precipitate the cluster transition (Koel et al., 2013 ).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com