If necessary, sections were cut for analysis by fluorescence in situ hybridisation using selected probes (from IGH, CCND1, BCL2, MALT, MYC; 4 cases).
If necessary, sections were counterstained for nuclei with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; 1 : 1000; Roche, Mannheim, Germany), and then mounted in ProLong Gold antifade reagent (Invitrogen) before imaging.
When necessary, sections were also stained according to Warthin-Starry for better visualisation of H. pylori.
Unnecessary C-sections involve medical risks for mothers and infants [ 4, 5] and also take away medical resources from necessary medical care, including medically necessary C-sections for other women.
The company argues that this is necessary to personalise comment sections for each viewer: "You'll see posts at the top of the list from the video's creator, popular personalities, engaged discussions about the video, and people in your Google+ Circles," Google explained in a blogpost.
If necessary, the sections were heated for 15 min at 90 °C in a microwave oven for antigen retrieval, and endogenous peroxidase activity was blocked by immersion in 3% (vol./vol)./vol
Larger cross sections for transporting the air are necessary.
Symbolic execution unrolls loops, hence, for most cases, neither loop bound annotations nor complementary loop bound analyses are necessary (see Section 5 for examples) except when the bound is dependent on, for example, I/O or the unrolling exceeds the specified analysis depth.
This is a natural starting point and this section contains necessary knowledge for the following sections.
Moreover, the analysis of [14] shows that the Dörfler marking (2.5) is not only sufficient to guarantee contraction (3.4), but somehow even necessary, see Section 4.5 for the refined analysis which avoids the use of efficiency (1.4).
