In polygynous mating systems with very high mating skew, directional female mate choice for "good genes" should deplete the male genetic variance that is necessary for indirect genetic benefits to be maintained.
Nevertheless, no direct genotyping of the FMR1 CGG expansion was necessary, since indirect analysis through the STRs was informative enough to determine the status of each embryo for the disease.
Finally, quantitative genetic studies estimating the genetic correlations necessary for indirect selection to occur (i.e. genetic correlations between female preferences and male display traits, and male display traits and offspring fitness) have also provided mixed results [ 33, 45, 46], although data of this type are sparse.
To attain your goals, it's sometimes necessary to employ indirect means.
Between-array normalization becomes necessary when seeking indirect sex-specific comparisons between tissues or loci.
All items within this construct showed low intercorrelations, in accordance with Azjen's [ 35] premise that internal consistency is not a necessary feature of indirect measures.
In cases where direct determination of the network is difficult or impossible it is necessary to use indirect evidence which can be more easily obtained.
It was necessary to use indirect immunofluorescence microscopy with fixed cells to detect the mRFP fusion protein in Dictyostelium cells because the mRFP fluorescence was too weak to detect with laser power that did not kill the cells.
On the other hand, those dimensions that we are able to modify, such as caregiving knowledge, lack validated questionnaires, which impedes gaining further knowledge and makes it necessary to employ indirect measures such as patients´ readmissions or life expectancies.
It is therefore often necessary to use indirect evidence to deduce the most likely structure of the nearly 55% of CDS structures that are not fully confirmed by transcript data.
