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In this case, the main disadvantage is the limited number of conditions that can be compared simultaneously (usually two), and also the large culture time needed to obtain the fully labeled cell populations that are necessary for quantification.
In both methods, arterial input function (AIF) is necessary for quantification.
At these concentration levels ICP-OES does not provide the precision necessary for quantification (at least 10%).
Also, a RIMS-set, i.e. one of each target, is necessary for quantification.
However, comparison with a more complex model would be necessary for quantification of model error.
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In our understanding risk is an interdisciplinary concept that acknowledges the benefits and necessary cases for quantification, but at the same time underlines the integration of individual perception and social construction.
To obtain the high-contrast tomography dataset necessary for structure quantification, a new image reconstruction and processing methodology was implemented.
Use of internal reference gene(s) is necessary for adequate quantification of target gene expression by RT-PCR.
Recalibration of ST-segment measurements may be necessary for meticulous quantification of ischemia, infarct size, or other measurements that might be affected by variations < 100 microV.
Fingerprinting techniques, such as ESI-MS, may be applied to sample characterization and the determination of plant origin of samples, but chromatography remains necessary for compound quantification.
Therefore, unless a better equilibrium can be achieved with a different technique, an arterial input function will be necessary for the quantification of [11C]befloxatone binding.
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