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Exact(5)
In the park the necessary amplification takes its toll.
There are lots of examples of this necessary amplification being done well: Budweiser's sponsorship of the Fifa World Cup gives it the right to choose the man of the match.
The detection step was estimated to cost $0.30 per patient; however, this cost does not include the cost of the necessary amplification step.
As C100 populations do survive, but initially show no visible growth, the necessary amplification event is even less likely, providing a further explanation for the lower abundance of resistant populations and the different waiting times.
The various RBS sequences (Table 1) for each gene construct were introduced with PCR primers if necessary (amplification utilized high-fidelity Phusion DNA polymerase from NEB and an Eppendorf Mastercycler gradient thermal cycler).
Similar(55)
When necessary, genomic amplification of DNA was performed by using the Amersham GenomiPhi kit.
Note that 1M Betaine was necessary for amplification of the H19/IGF2 ICR.
This involves a complex cascade and network of interacting proteins which is necessary for amplification and regulation of the process (for a review see Koonin and Aravind, 2002).
Alternatively, an intact, high molecular weight DNA necessary for amplification of large fragments was isolated by the salting out protocol [ 21].
However, Schwartz et al. [ 36] recently reported that palmitate metabolism to ceramide was necessary for amplification of LPS-induced inflammation in human monocytes.
The sample is placed in a well with a primer specific for the DNA sequence to be measured, necessary for amplification to begin [ 2].
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