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An optimum aliquot (10%, volume of inoculum/volume of growth medium) of preculture was harvested aseptically during the exponential growth phase (OD value ∼0.473 at 680 nm) and it was transferred to the fresh media (100 mL) supplemented with arsenic [either As III) or As V)] of necessary amount for different studies in 250 mL Erlenmeyer flasks.
The total greatly exceeds the necessary amount for our fitting procedure, yielding more robust and temporally smooth profiles.
Similar(58)
Compared with necessary amounts, the consumption of Asn, Thr, Ser, Gln, Arg, Ala, Val, Leu, Lys, His, and Phe exceeded the necessary amounts for growth of S. thermophilus T1C2 remarkably.
We have documented in a phase I study a safe administration of the full IT, which can easily be produced in the necessary amounts for clinical use.
Even if it moves in the direction traced by Chester [13, 14, 15], it aims to consider also the necessary amount of energy for dismantling and recycling the materials of the vehicle, opening the road to a better LCA management.
Biological materials were obtained directly from surgery after removal of the necessary amount of tissue for routine pathology examination.
Urinary bladder tumour specimens (stage Ta) were obtained after removal of the necessary amount of tissue for routine pathology examination.
Buy the necessary amount of wood for your bench project.
Prepare the buffer for perfusion step II by adding the necessary amount of Collagenase P for the digestion of the liver tissue to 100 ml perfusion solution II (pre-warmed at 37 °C).
One barrier to genome sequencing is the need to have a sizable inbred population in order to obtain the necessary amount of nucleic acid for sequencing while maintaining the homogeneity necessary to facilitate whole genome assembly.
Solid-state incorporation was performed by a modified procedure of Refs. necessary amount of dealuminated zeolite for 10 min in a pestle and mortar.
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