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Six lots of blank biological matrices were extracted each in triplicates and postspiked with the aqueous standard at the mid-QC level and compared with neat standards of the same concentration in alternate injections.
The efficiency of carbinoxamine enantiomers and IS extraction from human plasma was determined by comparing the responses of the analyte extracted from six reproduce QC samples with the response of analyte from neat standards at equivalent concentrations by a liquid-liquid extraction process.
The results of the comparison of neat standards vs. human plasma-extracted standards were estimated for CA enantiomers at low (300 ng/mL), medium (1200 ng/mL), and high (5000 ng/mL) levels, and the mean recovery for (d) and (l) enantiomers of CA was shown in Table 4.
Retention times were determined by comparisons to neat standards as well as by co-elution with standards.
Analytes were quantified by external standard quantitation using neat standards to calculate response factors based on the peak area of the analyte.
Similar(55)
Initially, each compound was prepared from a neat standard at a concentration of 1 μg/ml in pure methanol and injected into the mass spectrometer at a flowrate of 500 μl/min.
The matrix effects were assessed by comparing the responses of analyte and I.S. obtained from the extracted blank water samples (HPLC grade, tap water and river water) spiked with known concentrations of stanozolol and stanozolol D3 after extraction to those obtained from neat standard solution at the same final concentrations.
Pesticide residues extracted from comb samples were quantified using matrix matched calibration standards of known concentrations prepared from neat standard reference material.
In the case of fumonisins, loss of signal intensity in neat standard solutions in microinserts occurred over time.
Linearity was evaluated on neat standard and spiked matrix curves by fitting the data with a linear regression model.
Calibration curves constructed based on neat standard solutions appear to be linear within the range of 5 500 µg/L.
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