Hansen (2011) notes that there were many partial models (e.g. ASTRA, VACLAV, NEAC, SLAM and CG Europe) that were developed under the EU framework programmes for different scope and purposes, such as geography, mode, freight or passenger transport, economic models, demand models or network models.
We identified them as NEAC or NCC based on the specimen's pantophysin I (PanI) genotype, the accepted marker for distinguishing the two stocks [ 11- 13].
Individuals for the initial screen (ascertainment sample) were chosen in order to cover the major part of the distributional area of Atlantic cod (CAN, NEAC, CNS and BAS).
Star et al. [ 3] recently reported the annotated genome of a specimen (NEAC_001) from the cold-adapted North East Arctic cod (NEAC) stock from the Barents Sea.
Spatial analyses were conducted using samples from three different hierarchical levels; global (CAN, GRE, ICS, NCC, NEAC, ECH, CNS and BAS), a regional south-north transect of central and north-eastern Atlantic population samples (ECH, SNC, CNS, NNS, FBA, FPL, NCC NEAC, ICS, ICN and GRE) and a North Sea - Baltic Sea transect (CNS, KAT, BES, WBA, ARK and BAS).
Even lower frequencies of this allele were found in Icelandic coastal and frontal populations (7.8%), Northeast Arctic cod (NEAC) population in Barents Sea (3%), Faroe bank and plateau populations (1.5%) and in spawning NEAC fish outside Lofoten islands (1%), while the Met allele was not identified in the North Sea, Kattegat and Baltic Sea populations (Additional file 5: Table S4).
Atlantic cod G. morhua individuals of the Norwegian coastal cod (NCC) stock and North East Arctic cod (NEAC) stock were caught by trawl from the Finnmark coast and marginal Barents Sea sites.
SEF genotyped the NEAC and NCC individuals.
CHCC did the NEAC and NCC collection, genomic southern hybridization and codon usage analyses.
Therefore, each hybridizing band (TaqI fragment) in the genomic Southern blot represents one or more AFGP genes, indicating that a family of AFGP genes or highly similar sequences are present in the genome of both NEAC and NCC.
All NEAC and NCC individuals we examined showed multiple strong AFGP-hybridizing bands to an AFGP coding sequence probe derived from the related, fully AFGP-fortified polar cod Boreogadus saida[ 9], similar to the hybridization in the three polar cod individuals included as positive controls.
