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Concerning the NDT, in our protocol the sitting position was used as neutral position of the foot instead of palpating the talar head, so the ND could be determined directly and the measurement time was minimized.
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oMWCNTs and NDs could be distributed in different parts of the tissues in co-injection.
When increasing the amount of HCPT, NDs could be successfully manufactured (Fig. 2d).
Part of the NDs could be eliminated within 24 h after single injection.
oMWCNTs and NDs could be quickly cleared from blood post single or combined injection.
The biodistribution of NDs could be affected by oMWCNTs and depended strongly on the dose of MWCNTs.
The highest biodistribution of oMWCNTs was found in the lung tissues post injection with 99mTc-oMWCNTs or 99mTc-oMWCNTs + NDs (Figures 2, 4, and 5), and higher distribution of NDs could be found in the liver and spleen (Figure 3).
Meanwhile, oMWCNTs and a mixture of oMWCNTs and NDs could be mostly observed in the lungs post i.v. The blood clearance rate of nanoparticles was shown in Figure 12, and the results indicated that nanoparticles were eliminated almost completely from the blood after 2 h.
It has been suggested that a generally faster evolutionary rate in invertebrate ND genes could be due to positive selection [42], [43].
The Sm/Nd ratios of the Sm-doped Nd standard solutions could be reliably calibrated by the standard bracketing method mostly within 1% difference from the gravimetric values.
The drug amount difference in cellular uptake among the NRs, NDs, and NSs could be attributed to both the released drug and drug trapped inside the nanoparticles, no matter how much the ratio of PEG-b-PLGA to HCPT is.
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