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Aliquots (0.1 ml or ∼10 cells) were plated on selective medium (NCE, 1% lactose, Xgal, adenine, and thiamine) and plates were incubated at 30°.
Aliquots (0.1 ml or ∼10 cells) were plated on selective medium (NCE, 1% lactose, X-gal, andnine, and thiamine) and plates were incubated at 30°.
Aliquots (0.1 ml or ∼10 cells) were plated on selective medium (NCE 1% lactose, X-gal, adenine, thiamine) and plates were incubated at 30° (Yang et al. 2001).
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More distinct partitions can be found for smaller values of FPI and NCE [6].
Also, a good work on fast training of NNLM with noise-contrastive estimation (NCE) [25] was proposed in [26], where the normalizing factor for each context was treated as a parameter to learn during the training.
Cells were grown overnight on NCE 0.2% glycerol adenine medium at 37°.
A total of 3 million NCE (50,000 NCE in 60 mice) were scored at the microscope at 4 months of life.
MS2 analysis consisted of CID (quadrupole ion trap analysis, AGC 2 × 10, NCE 35, q-value 0.25, maximum injection time 100 ms).
'Top speed' (2 s) was selected for MS2 analysis, which consisted of CID (quadrupole ion trap analysis, AGC 4 × 10, NCE 35, maximum injection time 150 ms).
MS scans were acquired at a resolution of 17,500 with AGC of 2 × 10, maximum injection time of 120 ms, and stepped normalized collision energy (NCE) 50 ± 30%%.
MS3 precursors were fragmented by HCD prior to Orbitrap analysis (NCE 50, max AGC 1.5 × 10, maximum injection time 250 ms, isolation specificity 2.5 Th, resolution 30,000) (McAlister et al., 2012 ).
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