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NC: nucleus.
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The first signs of degeneration are disordered chromatin in NCs, followed by condensation of NC nuclei.
During late oogenesis, NC cytoplasmic contents are transferred to the oocyte during stage (st) 11, such that by st12, only NC nuclei remain in the anterior portion of the egg chamber.
13 During st13, NC nuclei become condensed and eventually disappear.
19 During early st12, punctate LT surrounded NC nuclei.
12 By st14, NC nuclei have completely disappeared in 93-95% of wild-type egg chambers.
Degenerating NC nuclei initially showed compacted chromatin, consistent with fluorescent imaging.
We found that all mutants examined showed a significant disruption in developmental NC death, displaying persisting NC nuclei at st14.
NC nuclei progressed to a phase in which the chromatin was highly dispersed with few surrounding organelles.
As seen in Figure 5d, CP1-GFP also clustered around NC nuclei during st12, partially overlapping with punctate LT.
Here we have shown that lysosomes cluster around NC nuclei during st12, and acidification of NC remnants is observed beginning in late st12.
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