Although naturally occurring restriction endonucleases are highly efficient at cleaving dsDNA at specific locations and have been used successfully to discriminate polymorphisms such as restriction fragment length polymorphisms (RFLPs), many SNPs are not associated with a natural restriction site.
This can be cloned into the pIB307 vector and restricted by endonucleases at naturally occurring restriction sites to replace the gene(s) to be deleted with the sacB-kan cassette.
The digestive products were separated by electrophoresis on 2% sepharose gel for 60 min. Both c.553G>T and c.457G>A are naturally occurring restriction enzyme sites in the exon 4 of ApoA5 gene.
The use of naturally occurring restriction endonucleases limited what could be achieved by gene therapy.
The lengths of the fragments were decided by using naturally occurring restriction sites within the promoter.
Either naturally occurring restriction sites were used or base changes were incorporated in one of the PCR primers to give a recognition site with one of the alleles of the polymorphic site.
However, with the reliance on naturally occurring restriction sites in the oligonucleotide sequence, and on additional oligonucleotides for ligation and cleavage, C2CA is more useful for signal amplification than for production of defined oligonucleotides.
This began with the creation of hybrid enzymes, composed of protein sequences called zinc fingers and an endonuclease domain taken from a naturally occurring restriction enzyme (Kim et al., 1996).
Differences in costs associated with water extraction between surface and ground water sources naturally impose a restriction to adopt irrigation.
They don't naturally come with restrictions on their use attached to them, and they don't naturally come with author information attached to them.
