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We have adapted a clone-FISH approach known from prokaryotes to optimize the hybridization conditions of a designed oligonucleotidic probe specific to Chytridiales (i.e. the largest group of the true-fungal division of Chytridiomycota), before application to natural samples using the CARD-FISH approach.
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Our results indicate that nucleobases can be easily isolated directly from natural samples using sublimation and then detected by GC-MS after chemical derivatization.
We estimated the proportion of CD4+ T cells, CD8+ T cells, B cells, natural killer cells, and granulocytes in each sample using the procedure described by Jaffe et al. [ 81] and adjusted all the polygenic regression models for these estimated cell counts as covariates.
Based on these observations, we made variant calls in the three natural viral samples using both 5×10-5 5×10-5more conservandve error rate of 5×10-4, where the formorerepresented the mean overall error rate and the latter approximated the Pconservativeerror
In this study a method for the determination of low total mercury (THg) concentrations in humic-rich natural water samples using cold vapor technique coupled to an inductively coupled plasma mass spectrometer (CV-ICP-MS) was developed and optimized.
A potentiometric stripping method for a direct measurement of cadmium in various natural water samples using a Stripping analyzer M1 (designed and manufactured in Serbia) unit has been developed.
We used the same stripping parameters for the detection of Mn concentrations in natural water samples, while using the method of standard addition to determine the concentration of Mn in the original samples.
The tomato natural population samples used consists of 92 tomato lines belonging to the core collection of EU-SOL and professor D. Zamir (The Hebrew University of Jerusalem).
THg concentrations in unfiltered, undiluted natural water samples determined using the optimized CV-ICP-MS method were compared with concentrations determined by cold vapor atomic fluorescence spectrometer (CV-AFS) based on the EPA method 1631.
Experiments were conducted with SLS samples, using natural soil and sand as controls.
Drug response phenotypes (IC50 values) for both drugs were adjusted for ethnicity, gender and storage time of the 197 samples using linear regression (natural log transformation applied to IC50 values).
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