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Although these applications are well-suited for the targeted activation of a single gene, genetic reprogramming requires the coordinated regulation of many nodes of natural gene networks as is typically performed by naturally occurring reprogramming factors.
Finally, four big pieces were put into yeast, which hooked them together using a natural gene repair mechanism.
As with GM food crops, there are fears about whether pharmed plants could breed with wild relatives and disturb the natural gene pool.
Transposons are natural gene delivery vehicles.
They can replicate only inside their host cell and can act as natural gene carriers.
However, the knowledge on mutations associated with natural gene polymorphism or resistance is limited.
Sacred groves are the natural gene pool preserver and example of habitat preservation through community participation (Gadgil and Vartak [1975]).
Risks of gene swamping or pollution of natural gene pools, leading to the extinction of wild taxa have also been stressed [14].
It was found recently that natural gene regulatory systems are governed by hierarchically canalyzing functions (HCFs), a special subclass of Boolean functions.
The use of multi-gene cassettes which mimic natural gene clusters can facilitate coordinated regulation of a pathway and speed transformation efforts.
The synthetic reconstruction of natural gene networks and the de novo design of artificial genetic circuits provide new insights into the cell's regulatory mechanisms and will open new opportunities for drug discovery and intelligent therapeutic schemes.
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