Sentence examples for native stability from inspiring English sources
The native stability of α1AT cavity mutants showed a negative correlation with the translocation rate but a positive correlation with the acyl-linkage hydrolysis rate, suggesting that the two kinetic steps are not independent but closely related.
However, the extent to which native stability itself plays a role, as opposed to specific mechanistic perturbations, has not yet been demonstrated.
Many mutations that alter the rate of heat-induced polymerization have been described, but interpretation is problematic because discrimination is lacking between the effect of global changes in native stability and specific effects on structural mechanism.
Serpins rely on differences in the energy of two conformational states during the inhibition of a target protease, and residual inhibitory activity provides an alternative function-specific measure of native stability relative to the inserted six-stranded form of the protein (Table 3).
Thermal denaturation experiments, using CD or environment-sensitive fluorescent dyes, such as SYPRO Orange, give information about the native stability of a protein with respect to the unfolded state [ 53, 54], represented at its most basic by a transition midpoint temperature Tm.
Other protein misfolding scenarios in which heterotypic interactions stabilize the native complex might also be candidate systems to test this approach, provided that appropriate tags that do not modify the interaction and the native stability of the proteins can be developed.
Despite these variations, the superfunnel paradigm applies to all SEMs we have tested: For a majority of neutral nets across different models, there exists a funnel-like organization of native stabilities for the sequences in a neutral net encoding for the same structure, and the thermodynamically most stable sequence is also the most robust against mutation.
On the other hand, we also have a test on the native asymptotic stability of EE point, a result that was just enunciated by Aranda et al. [3].
In this study, we probed how mutational substitutions of streptococcal pyrogenic exotoxin A (SPEA), a model bacterial superantigen, affect native conformational stability and antigenicity.
The major structural transition can be affected by single amino acid substitutions converging to the "consensus" ankyrin repeat sequence, increasing the native state stability significantly.
Extensive mapping of the native structure, stability and folding rate in sequence space using lattice proteins has revealed organizational principles of the sequence/structure map important for evolutionary dynamics.
