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UV vis spectra suggested that Hb in the film could keep its native secondary structure.
The denatured state had extensive native secondary structure and was significantly compact and globular.
This choice preserves the native secondary structure but allows the tertiary structure to change.
UV-vis and FTIR spectroscopy demonstrated that HRP in the composite film could retain its native secondary structure.
This is in contrast to circular dichroism measurements which show only 20 25 % regain of the native secondary structure in the burst phase.
The first thermal transition of Cro K56-[DGEVK] involves the melting of major parts of its native secondary structure and is accompanied by the formation of dimers and non-native β-sheet structures.
Similar(31)
The results demonstrate that a complex interplay between native tertiary interactions, divalent ion concentration, and non-native secondary structure determines the ruggedness of the energy landscape.
Therefore, these persistent native-like long-range NOEs imply that despite severely-disrupted tight packing and populated non-native secondary structures, at least some structures in the conformational ensemble of the partially-folded V22-SH3 still have a rudiment tertiary topology similar to its native SH3 fold [18].
In contrast, the ellipticity at 222 nm of the CtxB monomers at pH 7.0 (Θ222 nm = −9856 deg.cm2.dmol−1), and at pH 5.0, (Θ222 nm = −8550 deg.cm2.dmol−1), underwent detectable changes indicating that the two toxin monomers had different and non-native secondary structures.
Thus, we propose that the kinetic intermediate features an intact acceptor stem and T-stem, and non-native secondary structure in other parts of the molecule.
These aggregates may consist of oligomeric complexes of non-native secondary structures and demonstrate poor solubility in aqueous or detergent solvents [ 3].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com