The second method involves the use of an introduced (thus non-native) reporter mRNA that provides a real-time, live-cell readout of protein production from translation.
Transgenic genotypes were produced using the proximal GPT for the MBP gene to drive expression of linked, non-native reporter transgenes for HSV-TK and lacZ galactosidase (lacZ; Landry et al., 1998; Xie et al., 2002, 2009).
Transgenic genotypes were produced using the proximal GPT for the MBP gene to drive expression of linked, non-native reporter transgenes for HSV-TK, τ-eGFP (τ-enhanced green fluorescent protein) and/or lacZ (lacZ galactosidase) (Landry et al., 1998; Xie et al., 2002; Jacobs et al., 2007).
In both cases (native and reporter strains) the discrepancy observed between the mRNA and protein levels probably resulted from intrinsic regulatory mechanisms.
Recently a synthetic GUS transgene, codon optimised for use in sugarcane, was shown to generate significantly higher levels of GUS activity than the native GUS reporter gene [ 21].
Under Armour made it for me," the 31-year-old Chicago native told reporters on Friday after training.
"If ever I wanted a horse to win for a horse, this is the horse," Repole, a Queens native, told reporters in the winner's circle.
Guard Chauncey Billups, the team's leader and a Denver native, told reporters that he had removed himself from efforts to convince Anthony to stay with the Nuggets, stressing that at this point, it was Anthony's decision alone.
We have a core group of full-time traditional and native digital reporters publishing and interacting with readers under their own names and brands.
To compare these results with previously published data [13], the same test proteins were also fused to the N-terminus of the native topology folding reporter GFP or superfolder GFP.
