Exact(11)
The CRISPR/Cas9 system has been used to reconstruct the genetics of these complex rearrangements at native loci while maintaining the architecture and regulatory elements.
Third, in collaboration with Watt Webb's and Warren Zipfel's Lab, we applied the first multiphoton laser-scanning microscopy to optically dissect Drosophila polytene nuclei and measure transcription factor dynamics at specific native loci in living tissue.
The kit is primarily composed of two mother vectors, co-transformation of linear DNAs into E. coli can simultaneously introduce two butanol synthetic operons into the chromosome and create two in-frame gene deletions at targeted native loci.
To chromosomally integrate tagged genes into the native loci on the genomic DNA, pYC6, pYC11 [59], and the hygromycin resistance gene containing plasmid were used.
Importantly, reintroduction of four of six MICOS genes at their native loci complemented the growth defect, validating the ∆MICOS strain.
Herein lies the key to the model, as the co-existence, however transient, of foreign and native loci within the same subcellular compartment allows gene conversion to occur.
Similar(49)
Deng, W. et al. Controlling long-range genomic interactions at a native locus by targeted tethering of a looping factor.
Regulation studies of promoters are ideal at their native locus, which do not require prior knowledge of promoter regions.
We demonstrate the usefulness of one of these FRT lacZ GmR plasmids to study the regulation of the fadAB1 operon in P. aeruginosa at its native locus.
The strains carried the mutated mreC genes in the mreC native locus, and a wild-type copy of mreC on plasmid pMEG4 under control of an IPTG inducible promoter.
Dual D1 strains (nS345P:eWT and nD170A:eWT) expressed a wild type (WT) D1 from ectopic and a damage prone mutant D1-S345PP, D1-D170A) from native locus on the chromosome.
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