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The bayonet method allows primary closure of a wound and rapid restoration of the native length of the limb.
The ability to perform optical and electronic single-molecule measurements simultaneously could help bridge the native length scales of each experiment.
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Our results indicated that the IC50s for native-length Mabs 1 and 3 were essentially the same as the parental Fabs by cellular assay (Table 3).
Furthermore, direct comparison of parental Fabs with native-length Mabs 9 and 10 revealed no differences in blocking SEB binding to MHC II by this assay.
In contrast to the results obtained with Fabs (Fig. 2A), the native-length Mabs 3, 9, and 10 inhibited 60 – 80% of T-cell responses up to 12 h after SEB (Fig. 2C).
Although it was plausible that Fc addition to Fabs 9 and 10 increased steric hindrance of toxin-TCR interactions, as reflected in the IC50 values, comparison of Fab with its native-length Mab revealed no differences in blocking SEB binding to MHC II.
We define the mismatch d as the difference between the ligand's native state length and the correct target's length, d = l0−sA.
Below we examine this dependence to find the optimal ligand, specified by its mismatch d (or its native state length l0).
Native, full length plasminogen has an unusual far-UV CD spectrum as can be seen from the figure.
We examined the bioactivity of RG27, an aptamer that targets the above-mentioned pocket, interacts with RasGAP SH3 in a HeLa cell two-hybrid assay, and interacts with the native full length RasGAP protein in a pull-down assay.
Chemical affinity is taken into account by assuming that the competing target B has only N−m interacting binding sites while the main one has N. We test the specificity of a ligand specified by a native state length l0 and a flexibility k.
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