Exact(36)
A native dataset was collected at NSLS beamline X6A on a crystal that diffracted to 2.2 Å resolution.
A native dataset was collected to a resolution of 1.5 Å at ESRF beam line (Grenoble, France) BM14.
The final resolution was extended to 1.7 Å using a high resolution native dataset and refined without NCS.
Following initial rebuilding using the SeMet data, refinement was switched to minimize against the isomorphous native dataset.
The refinement was carried out with CNsimulatedted annealing [36] and Refmac [37] alternately using the 2.0 Å methylated native dataset and resulted in Rwork value of 20.30% and Rfree value of 25.14% (Table 2).
Native dataset and a complete MAD dataset from single crystals of SeMet incorporated NBD94566 663 were collected at 100 K on beamline BL26B2 at SPring-8 using a Mar CCD 225 detector.
Similar(23)
SAD experiments were recorded at beamline X06SA/PXI (SLS, Villingen) and native datasets at beamline ID23-1 (ESRF, Grenoble).
Native datasets were collected at APS LS-CAT and processed using the software HKL2000.
For the native species dataset, the final solutions reached were not stable among runs due to the low number of data, even after increasing the number of random starts (parameter trymax) to one hundred.
The native crystal dataset was collected at a radiation wavelength of 0.9194 Å on a beamline BL17U at 100 K with an MX-225 CCD (charge-coupled device) (Marresearch, Germany) at the Shanghai Synchrotron Radiation Facility (SSRF).
Attempts with molecular replacement using the unbound Rev dimer structure or models of Rev-IIB RNA complexes as starting models with native diffraction datasets (collected at 1.116 Å) were unsuccessful.
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