Non-native cultured fish can impact the genetics of natural populations either indirectly by reducing population size through competition or directly through introgression between artificially propagated fish and wild populations.
In contrast, the expression levels of 'commonly expressed RPE genes' are consistently high in almost all RPE preparations (excluding ARPE19; dotted box, Cluster 1) and therefore are not substantially affected either by culturing or by the choice of model (fetal versus adult or native versus cultured).
Biochemical analyses of native and cultured articular cartilage explants demonstrated that growth factor-treated cartilage was significantly different to both immature and serum-free cultured immature cartilage explants in water content (P < 0.05, N = 6), dry/wet weight ratio (P < 0.05, N = 6) and hydroxyproline content (P < 0.05, N = 6), Table 1.
Firstly, the gene expression profiles of native and cultured odontoblasts were compared at large-scale to investigate the similarities and differences between the samples.
Since the previous and published results supported coupling between ENaC activity and actin filaments, we were interested in testing whether such a mechanism plays a role in physiologic regulation of the channel in native and cultured principal cells.
Thus, the results in Figures 3 and 4 demonstrate that the NPo of active ENaC within the apical membrane of native and cultured principal cells is tightly coupled to the actin cytoskeleton.
PMCA2 was not expressed in either native or cultured SCCD and virtually absent in stria vascularis.
There was no statistical difference in sGAG values within native or cultured explant sample groups.
Hump-like features were also present in native and cultured immature cartilages, but their appearance was reduced in native mature and in vitro matured cartilage.
In conclusion, we have described a specific gene signature of human RPE based on extensive analysis of native and cultured cells.
Following in situ hybridisation and/or immunohistochemistry, whole-mounted microdissected (ie, native) and cultured crypts were visualised by laser scanning confocal microscopy (Zeiss 510 META).
