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Results are also presented that illustrate GFP does not alter the cultivation characteristics of the target protein when compared to the native construct.
Fab 10 and its full-length native construct both provided the highest protection (68%) from toxic shock within the groups examined, followed by Fab 1 (42%) and Fab 9 (34%).
While the reporter is not precisely the same mRNA as the native construct, it yielded the same conclusion (a very large translational repression) and thus was in good agreement with the ribosome profiling result.
Our results suggest that some of the peptides may be behaving slightly differently in isolation and within a fusion or the larger native construct; however, clear thermodynamic trends were observed.
Expression of sec22 was detectable at transcript level in both strains, with the expression from the overexpression construct stronger, and from the native construct somewhat lower than in the wild type.
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Second, the article refines the native capability construct, splitting it into native "pull" and "push" capabilities.
Native apps construct a silo around themselves and operate in their own artificially constructed world.
Flies expressing a functional, GFP-tagged NOMPA protein from a native transgene construct show an extended patch of GFP labeling at each bristle, outlining the sheath and the cilium.
The non-normalized frameshift efficiencies for the native sequence construct in all biological replicates (n = 9) was 5.4 ± 0.9%.
Subcellular distribution of P5CR-GFP fluorescence was the same in overexpressing plants and in plants with the native promoter construct, although in the latter case the GFP signal was hardly discernible from autofluorescence signals in mature tissues.
The analysis of contribution by position again showed good binding for the motif in both native and constructed cases, and many unfavourable contributions in the context of non-native interactions (Figure S3).
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