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Lam, Y. C. et al. ATAXIN-1 interacts with the repressor Capicua in its native complex to cause SCA1 neuropathology.
Here, we show how such motif libraries can be incorporated into combinatorial sequence optimization protocols and improve native complex recapitulation.
By fluorescent labeling and confocal reconstruction we show that many astrocytes in organotypic hippocampal slice cultures exhibit a more native complex cytoarchitecture.
Thermodynamic stability of the native complex and a cooperative transition to the unique native structure suggest the nucleation kinetic mechanism at the equilibrium transition temperature.
Two potential cross-linked peptides (present in the cross-linked sample but undetectable in α, β, and native complex) were assigned.
The native complex (1DFU) is shown in cartoon.
Antiserum against the native complex of laminin and entactin also immunoprecipitated these polypeptides, although less efficiently.
Although controversial, the band is most likely a native complex defect of zinc vacancy involving Zni, OZn, and VO[26].
As shown in Table 3, the percentages of the interface areas in the native complex structures for the four cases are much lower than those in the top ranked structures.
The identification of endogenously expressed ISD11 in the native complex further confirmed the original co-IP experiments.
Recently, the mimicked interaction of CD4M33-F23 in complex with gp120 has been investigated in detail and compared to the native complex structure of CD4 and gp120 [38].
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