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Native cholesterol was measured at m/ z 458, and the deuterated cholesterol was measured at m/ z 464.
Bodipy-cholesterol has been shown to behave similarly to native cholesterol in cultured vertebrate cells (Hölttä-Vuori et al., 2008) and to partition into liquid-ordered domains of model membranes enriched in cholesterol and sphingomyelin (Shaw et al., 2006; Li and Bittman, 2007).
In each sample, the concentration of deuterated cholesterol was normalized to the concentration of native cholesterol, and the area under the curve of the deuterated cholesterol plotted against time was calculated by numerical integration using the trapezoidal method, exactly as described (17).
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The first pool is the 'PFO-accessible pool' that binds I-PFO* when the membrane is in its native cholesterol-replete state.
Although statins are well-known to reduce the native LDL cholesterol, reports related to the effect of statin therapy on the plasma Ox-LDL are rare.
Although statins are widely used in patients for the reduction of native LDL cholesterol, reports on statin therapy and plasma Ox-LDL are rare, and studies on the effects of statin therapy on Ox-LDL and their association with clinical outcomes of stroke are limited.
2. Substrates of native LDL-cholesterol and modified LDL-cholesterol.
The mechanism of the rise in plasma Ox-LDL after acute cerebral infarction remains unclear, although it is speculated that AIS is associated with enhanced oxidative stress that can further oxidize native LDL-cholesterol to Ox-LDL [ 21, 22].
Acute ischemia leads to increased production of free radicals and reactive oxygen species (ROS) in ischemic tissue and plasma [ 3], and oxidative stress may further oxidize native LDL-cholesterol to Ox-LDL.
Conversely, asymmetric dimethylarginine concentrations are substantially elevated by native or oxidized LDL cholesterol [36].
Both native and oxidized LDL-cholesterol have been shown to up-regulate PRMT while causing a decrease in the activity of DDAH [ 18].
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