If the DNA template is crowded with trailing RNA polymerases, nascent RNA molecules will have difficulty in binding template DNA, disrupting R-loop formation (Fig. 1B).
One of the major reasons for the paucity of mammalian Pol II terminator sequences in the literature is that examination of termination mechanisms is hindered by the technical difficulty of mapping nascent transcripts in nuclear run on experiments (Proudfoot, 1989).
The challenge for new ventures is to 'carve out a new market, raise capital from sceptical sources, recruit untrained employees, and cope with other difficulties stemming from their nascent status' (Aldrich and Fiol 1994, 645).
His handpicked successor, Nicolás Maduro, was elected by a slim margin in April and since then has confronted political turmoil and serious economic difficulties that have tested his nascent leadership skills.
Major reasons for this are the considerable technical difficulties involved in the analysis of nascent Pol II transcripts and the fact that Pol II termination has not as yet, been recapitulated in vitro.
While this isn't a glowing endorsement of the nascent currency, it's worth noting that economists occasionally have difficulty predicting events, such as the massive recession that almost tanked the global economy.
Flikshop experienced the very same difficulties in finding initial support; Bullock ended up funding his nascent company out-of-pocket.
That can stymie nascent breakthroughs.
"All these difficulties have clearly had a negative impact on the effectiveness of the judicial system, at a time when East Timorese confidence in the nascent judicial system is vital," Mr. Annan said.
Navigating these nascent sites is fairly straightforward.
Take Europe's nascent bond market.
