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YAC Y53A2 complemented the Bah phenotype, further narrowing the interval to approximately 350 kb.
Assuming the same functional variant in the Swedish and the Finnish samples, the two samples would assist in narrowing the interval of that functional variant (Fig. 2C).
The genetic deficiency hDf17 failed to complement bah-1 [16], narrowing the interval containing bah-1 to approximately 4 map units on the right arm of chromosome I.
Mapping a QTL to an interval of 20 60 Mb has become a relatively routine matter for traits with at least moderate heritability, but narrowing the interval to a small region that includes only a few candidate genes (1 5 Mb at most) is still a highly challenging task.
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Fine mapping confirmed and narrowed the interval and two genes, THBS2 and SMOC-2, were implicated.
We have also begun the process of creating nested congenic lines to further narrow the interval for the hit on chromosome 11, our most robust finding.
To further narrow the interval, primers and probes flanking the SNPs in the linked region were designed in multiplex format using SpectroDESIGNER software (Sequenom, San Diego, CA).
Reiterate steps 4e f to narrow the interval until all recombinants are exhausted or the interval is small enough to proceed to step 5 (see criteria below).
If there are more available polymorphic SSRs within the region defined by the flanking markers, use these to genotype only the recombinants and further narrow the interval.
If polymorphic, any interesting candidate that is sequenced can also be used as a new marker to narrow the interval in the mapping population.
k s k (20 ) 2 is therefore useful for detecting anomalous areas of the genome; however, a high-resolution scan of k s k (1 ) 2 values can be used to subsequently narrow the interval of interest.
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