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After 12 rounds of dialysis with 3-µm-pore-size membranes, vesicles smaller than 3 µm in the population were almost entirely eliminated, resulting in a relatively narrow population size distribution between 3 and 5 µm in diameter (Figure 2C and 2D).
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Patients were enrolled from a single center, thus narrowing the population size and the applicability to larger populations.
Even in clinical trials, where many limitations like that of limited size, narrow population, narrow indications and short duration are observed, we also found that evaluation of drugs on environment is practiced very minimally.
Due to narrow distribution range, small population size and high use, the species appears among the 37 identified as top priority species for conservation and cultivation in western Himalaya.
This estimate was then tripled to determine a target number of samples to be collected within that area, since at least three times the number of samples as expected individuals has been recommended to achieve a narrow confidence interval for population size estimates using mark-recapture methods [ 33, 43, 44].
27 The precision of the estimate could be narrowed with a larger population size.
Slower LD decay was observed within sub-groups, ranging from 1.5 cM (North America-Europe) to 6.0 cM (South America), which could be attributed to the limited population size and narrow genetic diversity of some sub-groups as compared to the core collection.
Considering that asthma prevalence estimates are more reliable for the U.S. states and regions with larger population size, having narrower 95% confidence intervals [16], and that the Eastern Seaboard is home to most highly populated U.S. cities, a regression analysis based on the data from this region is expected to be more reliable when compared to the data for all continental U.S. regions.
Ongoing selective breeding and narrowing of the breeding pool may have also reduced effective population size in some domesticated lineages (Medugorac et al. 2009).
For instance, Rabinowitz [ 3] and Rabinowitz et al. [ 4] suggested categorizing plant species according to geographic range (large or small), local population size (large or small) and habitat specificity (wide or narrow).
According to Rabinowitz' scheme (1981), in which species were classified into categories according to their geographic range, habitat specificity and local population size, L. multiflorum can be classified as a 'classic rarity' since it presents both narrow geographic range and narrow habitat specificity, thus being considered a restricted endemic.
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