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One important parameter is the selection of an appropriate plane of focus.
Videos were rejected if the plane of focus altered significantly during imaging acquisition.
The color coded emission profiles corresponded to one acquired plane of focus.
Typically apoptotic bodies are best seen at a different plane of focus (above) normal cells.
Variables included the plane of focus, incident excitation power and dispersion compensation.
Thus, we selected a plane of focus that was slightly anterior to the photoreceptor layer.
This narrowing of focus is, nevertheless, unfortunate.
This week I felt like there was a narrowing of focus.
Hypnosis involves selective attention, a narrowing of focus and an increased absorption in suggested experiences.
The long, narrow planes have engines mounted to the rear of the fuselage and the tail high in the air.
An optimal pinhole size of 60.64 μm was used to exclude fluorescence light emitted from out-of-focus plane above and below the focusing plane.
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