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Sequencing of DNA was done by Mission Biotech (Nankang, Taipei) with the same primers as for the PCR.
Mice (6 8 week old male C57BL/6JNarl or B6) were purchased from the National Laboratory Animal Center in Nankang, Taipei, Taiwan.
Random phone numbers were generated by a computer system, developed by the Nankang IT Company specializing in survey and data collection.
Briefly, two pLKO.1 lentiviral vectors containing different shRNA sequences (5′-CGCTACTTGACATTGGACCAA-3′ designated as sh1; 5′-GCCAATCGTTCT CTGACAGAA-3′ designased ash2h2) targeting FoxM1 mRNA were purchased from the National RNAi Core Facility Platform (Nankang, Taipei) and were used to generate recombinant virus for infection.
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