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This spiking pattern places naive neurons within the gray-shaded region of our excitability diagram.
To determine whether α-syn mAbs can prevent cell-to-cell transmission of pathologic α-syn aggregates in this chamber system, we added Syn211 or Syn303 to C2 30 min prior to adding pffs to C1, where the transfer of pathologic α-syn from affected neurons to naive neurons would be expected to occur.
These data confirm that bursting would have co-developed with repetitive spiking and MPOs if adaptation currents had been present, and suggest that adaptation currents are upregulated after nerve injury (possibly as a compensatory measure) rather than existing occultly in naive neurons whose spiking is already transient.
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10.7554/eLife.02370.005 Figure 2. Reproduction of neuropathic excitability in naive cutaneous neurons.
In the injured and naive DRGs, most neurons expressed L1-CAM mRNA, and weak signals were also observed in satellite cells and Schwann cells.
We have tuned the model to g syn= 0.0035mS/cm for a first spike for the naive or standard neuron (all µ = 1).
To avoid the effect of CFA-induced inflammation on the PVN neurons, 3 naive rats given EA treatment and 2 untreated naive rats were used in this experiment.
Using neurons from naive rats, we subsequently verified that muscle afferents, like cutaneous afferents described in Figure 2, normally exhibit onset-only spiking (n = 27 muscle afferents).
Intratracheal application of capsaicin (300 pg in in 20 μL) induced an increase in the firing activity of the airway-related NTS neurons in naive rats, which was maximal (9.9 ± 0.7 spikes/s) 20 min after the administration of capsaicin).
A seductive but naive idea is that single neurons (or tiny neural bundles) might be devoted to the representation of each thing the brain needs to record.
NaV1.8 or NaV1.9 AS ODN treatment generated marked loss of NaV1.8- or NaV1.9-positive DRG neurons in both naive and CFA-treated groups (Figure 3A).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com