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Relative to naive brains, we found that NF-κB p65 expression was significantly elevated at both RNA and protein levels at all timepoints examined, with the highest levels seen at day 14, several days past the point at which infection was no longer detectable (Fig. 1b).
You see, I knew what to expect, thanks to a jaunt up to the the Fiera del Tartufo a while ago with my friend Charlotte where our naive brains were dulled by the seductive yet suffocatingly sweet scent.
For investigation of the brain architecture in wild-type and Bmf −/− mice, naive brains were also prepared.
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These results suggest that blue light itself is capable of increasing CBF, in naive brain tissue.
Christie, I. N. et al. fMRI response to blue light delivery in the naive brain: implications for combined optogenetic fMRI studies.
At high magnification, RFP expression was found in occasional perivascular cells in healthy Ccr2RFP/+Cx3cr1GFP/+ brains (Figures 5A and D); flow cytometry, these RFP+ cells accounted for <2% of the CD45+ marrow-derived cells in naive brain (data not shown).
This mimics the naive brain, where the NG2+ population are the most proliferative cell type in the brain parenchyma (Gensert & Goldman, 2001; Dawson et al., 2003).
A graphical representation of our proposed hypothesis based on our observations that only a subset of NRSE-containing genes are functionally repressed by seizure-induced increases in NRSF levels and that these genes appear to have moderate NRSF binding in the naive brain.
a, Coronal section of naive rat brain.
Figure 1: Blue light generates a rapid increase of cerebral blood flow (CBF) in the naive mouse brain.
Combining two-photon laser scanning microscopy and ultrafast functional ultrasound imaging, we report that in the naive mouse brain, light per se causes a calcium decrease in arteriolar smooth muscle cells, leading to pronounced vasodilation, without excitation of neurons and astrocytes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com