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Electrocardiographic recording was possible on spontaneously beating artificial myocardial tissue samples and revealed physiologic patterns.
Methods: Artificial myocardial tissue samples were engineered by seeding neonatal rat cardiomyocytes with a commercially available 3-dimensional collagen matrix.
The following day, the microsphere reference blood samples and the same myocardial tissue samples were again counted for 5 min each.
Left ventricular myocardial tissue samples from 7 patients with end-stage heart failure were examined with transmission and scanning electron microscopy.
The serial blood samples and myocardial tissue samples were counted for 3 min each in the gamma well counter (Model 1282, LKB Instruments, Gaithersburg, MD, USA) with a window setting of 120 to 160 keV to detect Tc-99m activity.
Elution of hydroxyproline from myocardial tissue samples was verified and quantified by co-elution with known amounts of derivatized hydroxyproline standards (Fluka, Buchs SG, Switzerland).
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For myocardial tissue sample collection, animals were treated with 0.9% saline (vehicle) or 1 mg/kg BW373U86 administered via the right jugular vein for 10 minutes prior to removal of the heart.
Since the mortality rate at 24 48 h after MI was high in OLETF at ages of 25 30 weeks [ 4], myocardial tissue sampling for biochemical analyses and immunohistochemistry was performed at 12 h after MI.
Although Isaaz et al. already described pulsed Doppler recordings of myocardial tissue motion using a simple sample volume technique in 1989 [ 2], little clinical value was awarded to this technique.
To maintain the homogenous characteristic of tissue between BSP and MassARRAY, tissue samples from the same myocardial region were used to examine the methylation level of upstream sequence of ALDH2 core promoter.
Among specimens (serum samples from 11 patients and myocardial tissue from 2 patients) tested for enterovirus and adenovirus by PCR and enzyme immunoassay, all were negative (Table 2).
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