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The coding region of MYBA is 100 and 98% identical to MYB1 and MYB10, respectively [ 15].
The loci have been named MYB1, MYB10 and MYBA [ 12, 15- 17].
Interestingly, the endogenous activity of the MYBa-mutated promoter was notably higher than that of the wild-type promoter, suggesting that the MYBa element might bind a repressor, at least in leaf mesophyll cells.
This GUS expression pattern was also observed with the MYBa-MYBb double mutant (see additional file 3), confirming that MYBa does not play a crucial role in the promoter activity under these experimental conditions.
In a collection of 32 unrelated cultivars, MybA and AOMT expression profiles correlated with the level of methylated anthocyanin.
These elements are named MYBa (-163 -156 in reverse orientation) and MYBb (-146 -139 in direct orientation).
In addition, MYB10 and MYBA have been mapped to the same region on linkage group 9 [ 15, 18].
Similar(4)
The BSa- or MYBa-mutated EgCAD2 promoters (see additional file 3) drove a GUS expression pattern similar to the wild-type -203 EgCAD2 promoter (Fig. 5a).
We use gene specific primers to show that the three MYB activators of apple anthocyanin (MYB10/MYB1/MYBA) are likely alleles of each other.
However, for apple, MYB10/MYB1/MYBA is the major gene in a crossed population segregating for red flesh [ 75] and red skin [ 38].
As we do not see DNA fluorescence consistent with a 1 3 amplification of the 1000- and 900-bp fragments, we propose that MYB10 and MYB1/MYBA are alleles.
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