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For comparison, the last column reports the maximum likelihood estimates obtained in a previous study based on the 2 MY calibration point but a fixed topology [ 28], with standard errors in parenthesis.
For comparison, the TMRCA ages for the same clades were calculated with a 2 MY calibration point, and compared with results from [ 28], where the 2MY calibration point and simpler statistical methods (based either on a fixed topology or on the pairwise estimator Rho) were used.
Colors were generally accurate after my calibration, but I couldn't get the black level as dark as I like it. .
Colors were generally accurate after my calibration, but I couldn't get the black level as dark as I like it.
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Steppan et al. [ 7] derived divergence estimates from a four nuclear gene concatenation, using a variety of different estimation methods and a 12 My fossil calibration point for the basal radiation of all extant Murinae.
Using the silent site divergence at the coding region of MtGS2a between these two species (K s = 0.2475), and the estimated age of 14.9 My as calibration point, it is inferred that the Medicago GS2 gene duplication (K s = 0.1668) occurred about 10 My ago.
Yet according to the calibration, my set was now, as Video Essentials puts it, "100percentt correct".
Regarding the problems that Samadani et al. point out in terms of spatial calibration, my intent is not to dispute the existence of alternatives to eye movement assessments based on spatial calibration.
The ages inferred with the two calibrations differ little even for the hummingbird crown age (the deepest node), which is 24 (20.9-28.1) my using the fossil calibration or 25 (23 27) my using the rate calibration.
The differences reflect their use of a different calibration (10 My for Mus/ Rattus divergence) combined with a fixed-rate molecular clock.
We used Modeltest to estimate the model of nucleotide substitution and calculated the number of substitution using the origin of the family at 32 My ago as a calibration point as implemented by Teeling et al. [ 52].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com