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Furthermore, the Spc1 kinase and Atf1 protein are mutually required for a broad range of diverse stress responses, including the induction of sexual differentiation (and hotspot recombination) when nutrients are limiting (e.g., [12] [14], [25] [36]).
However, we now show that the HIKM complex is mutually required for stable association of CENP-T/W with kinetochores.
In prior work, we showed that Dis3 and Rrp6 physically interact and co-localize in S2 cells and are mutually required for proper localization [ 13].
Furthermore, GFP-CENP-ML94A L163E did not localize to kinetochores, indicatinGFP-CENP-ML94A L163ENP-I GFP-CENP-ML94A L163Edidor kinotochore localizetoon.
Moreover, we provide evidence that both SMC3 and MED12 colocalize on the ESR1 gene and are mutually required for their own occupancy as well as for RNAPII occupancy across the ESR1 gene.
Reviewer 1: The authors use point mutations in GFP-tagged CENP-M to suggest that 'CENP-M and CENP-I are mutually required for kinetochore localization', as mutant CENP-M does not localize to kinetochores or rescue CENP-I localization.
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Instead, emerging studies from other systems emphasize the necessity of mutually inhibitory circuits required for complex cellular outcomes (Tyson et al., 2001).
The model discussed above takes a more integrative perspective by viewing the evolution of ecological differentiation and the evolution of assortative mating are mutually dependent processes that are both required for adaptive speciation.
Fast diffusion recreates the mutually uniform spatial concentrations of the reactants required for mass action to hold.
We thus propose that CDK11 is required for centriole duplication by two non-mutually-exclusive mechanisms.
However, in ap, lms double mutants the majority of LT muscles are still present as well, thus ruling out that the two genes are required for LT muscle specification in a mutually-redundant fashion.
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