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While recombination can bring together multiple mutations within an organism, evolution in asexual populations depends on the sequential accumulation of mutations within a lineage.
Using dCas9 to recruit a hyperactive variant of the deaminase AID, we can target diverse point mutations within an ~100bp window centered on the sgRNA PAM site.
NOD mice are an inbred Swiss strain that harbor mutations within an ortholog to the human T1DM-susceptibility locus and therefore share key pathological hallmarks with human T1DM.
This information would contribute to our understanding of how the diagnostic mutations within an Alu source gene affect its retrotranspositional success.
Thus, mutations were not assigned to individual plaques; rather, all mutations within an individual mouse were identified by alignment of each read against the lacZ gene.
Laboratory studies focusing on genetic exchanges between microbes have described the recombination of mutations within an evolving population (Winkler and Kao, 2012) or the replacement of an endogenous metabolic pathway with an alternative route (Chou et al., 2011).
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In both of the experiments, as in Fig 2B, the mutated primer contained two mutations within a 3 bp region.
Statistical analyses of mutational biases were performed by pooling microsatellite mutations within a given vector observed among all cell clones, followed by Fisher exact test (two-sided).
Two gene copies (clones 3 and 4) harbored mutations within the A-box regulatory element.
Studies of mutational composition of individual cells showed that accumulation of mutations within a cell usually does not occur via accrual of random hits.
Rarity of clonal mutations within a region does not, however, rule out selection in that region.
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