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ENU mutagenesis is another powerful technique that can be used to produce allelic series of mutations with differing effects upon function of single genes (e.g., Andrews et al., 2012).
The sequencing of mutIV-mix-2 also show various mutations, with differing percentages.
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In this study, we developed an experimental model in which the actual human coding sequences of TGFBR2 exon 3 and ACVR2 exon 10 were evaluated in real time for −1 bp frameshift mutations in human cells with differing MMR genetic backgrounds (hMLH1−/−, hMSH6−/−, hMSH3−/−, and MMR-proficient).
Furthermore, although MAPT and GRN mutations have been associated with differing clinical and neuroanatomical features, a number of studies have highlighted a high degree of variability within subjects with each of these mutations, as well as within subjects, within the same family (van Swieten et al., 1999; Janssen et al., 2002; Boeve et al., 2005; Kelley et al., 2009).
Here we address these issues directly through a simulation approach designed to mimic situations arising in empirical datasets by combining loci with differing mutation rates.
The difference in p53 mutation location between PG and NPG carcinomas may suggest differences in function correlating with differing morphologic development.
They fail to: (i) account for multiple bacterial subpopulations with differing susceptibilities; (ii) quantify or interpret the explicit interaction (synergy/antagonism) mechanisms; and (iii) accommodate spontaneous mutations.
I struggle with differing opinions.
Works with differing viewpoints were often prohibited.
Furthermore, primary cells cultured from patients with FGFR2 mutations and mice generated with the same mutations show differing proliferation and differentiation characteristics (reviewed in [ 22]).
Finally, clones with rpoB mutations differed in phenotype from those with rho mutations, largely due to differences in chemical sensitivity.
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