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Instead these mutations will involve combinations of many small and partially compensating long-range interactions.
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Most mutations will not involve coding genes and will have no selective effect.
The best approach for detecting mutations outside the hotspots will involve direct sequencing of the polony PCR products [ 2, 13, 14].
Future experiments will involve mutation of this amino acid and correlating it with GmRLK18-1 stand/ory and/or function in binding the CLE like peptide motifs found in all plants and their many of their pathogens [ 30].
While our scenario will involve screening for mutations of a heterologously expressed gene in S. pombe, the same approach can be used to identify mutations of interest in an S. pombe gene carried on a plasmid.
Thus if selection is sufficiently strong, subsequent sweeps are likely to begin before significant variation has accumulated within the population, greatly reducing the possibility that the second sweep will involve a double mutation.
This by necessity will involve mammary gland transplantation in order to confirm that the effects of the mutations are organ autonomous.
All attempts will involve uncertainty.
Enhancing productivity will involve structural reform.
Buying A.I.A. will involve heroic contortions.
Bigger investments will involve other perks.
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