Indeed, bacteria have the ability to rapidly accumulate beneficial mutations when growing in new environments [ 18].
To test this hypothesis, we determined the effect of isoform-specific daf-16 /FoxO mutations on life span in animals harboring a temperature-sensitive glp-1 mutation that develops without a germline when grown at 25° (Arantes-Oliveira et al. 2002).
But when combined with an etr1-7 LOF mutation, the etr1-7; ers1-2 double mutant displayed an ethylene response phenotype when grown in the absence of ethylene, this effect being more pronounced in light-grown plants than in dark-grown seedlings.
Supplementary Figure 1 Hypha that were induced by the chk1-hyp mutation become more resistant to washing when grown on EMM-2 agar media.
A phenomenon, that was also observed with other fem, aux or fmt MRSA mutants which still harboured their original mutations but regained resistance by compensatory events when grown in the presence of β-lactams [ 18, 49].
Heterozygotes for one weak missense mutation and one strong nonsense mutation in aux (auxK47/auxD128) [32] rarely reach adulthood when grown at 25°C (Table 1).
Mutations can certainly change cellulase production, for instance, when grown on crystalline cellulose, a significantly high amount of cellulase can be synthesized and secreted by a mutant fungus of T. reesei QM6a (mutant RUT-C30).
One promoter-replacement mutation, ΔPrfc174, yielded an optimal profile, exhibiting wild-type characteristics when grown with arabinose, and Δrfc characteristics when grown without arabinose.
Accordingly, we noticed that mutations in components of the Doa10 complex have a mild growth phenotype when grown at low temperatures (10°C), as previously reported (Loertscher et al., 2006 and data not shown).
