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Long polymerase chain reaction (PCR) primers containing the mutations were used and the mutated fragments were cloned in the SMG4-gal4 vector.
Only those without any unintended non-synonymous mutations were used for the subsequent DNA transfection experiments.
These non-synonymous tumor mutations were used to predict potential tumor neoantigens for MHC class I alleles.
DNA from 115 unrelated test samples was pooled and samples with known mutations were used as positive controls to assess the sensitivity of our approach.
Wild-type GFP-muskelin and a panel of alanine point mutations were used to test the sensitivity of self-association to PKC activation.
A standard group of 15 samples with previously known DNA sequences and a test group of 37 HPA patients with unknown mutations were used for assay validation and application, respectively.
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Therefore, in addition to chromosomal alterations, FGFR3 mutations are used as a clonal marker.
An isogenic strain lacking the mutations was used as a wild-type control.
KRAS mutations are used as negative predictors of antiEGFR therapies in colorectal cancer [ 38].
In this study, either three or four iPSC lines from healthy controls and two lines from patients carrying the SOD1D90A mutation were used.
Crossover and mutation were used to generate individuals that represented new potential solutions.
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