Sentence examples for mutations were transformed from inspiring English sources

Site-directed mutations of AbOPH were constructed using QuickChange® Site-Directed Mutagenesis Kit (Stratagene, USA), and the plasmids carrying the gene of AbOPH and the desired mutations were transformed into E. coli DH5α for amplification and then transformed into E. coli BL21 DE3) cells for expression.

These mutations were transformed into Z5463, Z5463PI or Z5463BC as needed.

The AtLYK5 wild-type gene or versions with specific point mutations were transformed into Atlyk5-2 mutant plants.

The resulting plasmids containing ISCU2 with the desired mutations were transformed into BL21 DE3) cells and grown at 37 °C until the OD600 reached 0.6.

Clones confirmed by sequencing to contain the targeted mutations were transformed into E. coli KS330 for optimal protein expression, and are referred to as P66M-D205A,D207A P66M-Del202 208208. P66M-Del202 208

Diploid yeast strains heteroallelic for ade2 or leu2 mutations were transformed using linearized CFVs that invade upstream of the ade2 or leu2 loci and the resulting transformants were tested for adenine or leucine prototrophy, respectively.

After temperature cycling and treatment with DpnI to digest the parental DNA template and select for the desired DNA construct, the nicked vector DNA incorporating the mutations was transformed into E. coli.

An A. nidulans strain that contained the riboB2 and creB1937 mutations was transformed with pPL3 containing riboB +, with pTRcre2, containing T. reesei cre2 also present in the transformation mix.

The reaction was placed on ice for 2 minutes, 1 μl Dpn I (10 U/μl, New England Biolabs, Ipswich, Massachusetts, USA) was added, incubated at 37°C overnight to digest the parental (i.e., the non-mutated) plasmid template DNA [ 17] and the recircularized vector DNA incorporating the desired mutations was transformed into competent DH5α E. coli.

Once a selectable secondary phenotype was determined, cells containing the suppressor mutation were transformed with a genomic library in YCp50 and transformants were screened for rescue of the secondary phenotype.

Plasmids containing the proper mutation were transformed in electrocompetent JM109 DE3) cells and stored at −80 °C for long-term storage.