Mutations were transferred by P1 transduction (Miller, 1972).
Of these, only the 23S rRNA and spr1021 mutations were transferred to R6T2 and/or 1974T3 (Table 2).
Of the fifteen mutations identified in the 1974M1 mutant (Table 2), six mutations were transferred into both 1974T3 and R6T2 transformants (Table 2).
rol-6; nrde-1 animals were crossed with nrde-1 / dpy-17 males, F1 males were crossed with mjIs144; dpy-17 hermaphrodites, and F1 with both marker mutations were transferred for several generations to obtain strains that lacked Rol or Dpy mutations, which were mjIs144; nrde-1.
prg-1; rol-6 strains were crossed with prg-1 / unc-13 males, and F1 males were crossed with unc-13; mjIs144 hermaphrodites, and F1 with both marker mutations were transferred for several generations to obtain strains that lacked Unc or Rol mutations, which were prg-1; mjIstrainsrainstrains
Therefore, the 15 point mutations were transferred to either TetA(B -His10 (G44V, G62V, R70G, R101G, R101V, P227V, D285A, D285G, D285V, G336V and G346V) or TetA(B -His1001-Thrombin-His12 (loop deletions and D66A, D66G62V214G and W369G) foR70GR101Gation and ITC measuR101Vts (Fig. 4, Table 1 and SuP227Ventary Figs. 1–5).
To identify which mutation or combination of mutations was transferred from M184 to the three transformants to give patAB overexpression, the whole genomes of the transformants were sequenced by Illumina sequencing.
If the amplified copies (with or without novel mutations) are transferred to new recipient cells, this could lead to a higher level of sequence diversity for genes located in the amplified region.
To date, the only therapeutic option for preventing the transmission of mtDNA mutations is transferring embryos below the threshold of clinical expression based on preimplantation genetic diagnosis (PGD) (Brown et al., 2006).
Each mutation was transferred to the nuo operon expression vector, pBA400, and the resulting plasmids were used individually to transform the nuo deletion strain BA14.
The T45A-D46A mutation was transferred from the suicide vector down to the virulence plasmid by conjugation and double cross-over performed using previously reported methodology [65].
